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生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

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生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用

10,051 Views

•

12:25 min

•

September 28, 2018

DOI:

10.3791/58393-v

DOI:

10.3791/58393-v

•

12:25 min

September 28, 2018

•

1 Views

Anthony Asher¹, Iniyan Ganesan¹, Laura Klasek¹, Steven M. Theg¹

¹Department of Plant Biology,University of California – Davis

Summary

本文提出了用于叶绿体双精氨酸易位 (cpTat)、分泌 (cpSec1) 和信号识别粒子 (cpSRP) 通路的生理活性类囊体和蛋白质转运测定的高产量隔离的协议。

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