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Biologia do Desenvolvimento
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Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

DOI:
10.3791/58339-v

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06:58 min

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October 22, 2018

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, , ,
1Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Capítulos

  • 00:04Título
  • 00:505-ethynyl-2′-deoxyuridine (EdU)-labeled Bacteria Preparation and C. elegans Feeding
  • 02:46Germline Dissection, Fixation, Rehydration, and EdU Click Reaction/Detection
  • 05:09Results: Representative C. elegans Cell Cycle Analyses
  • 06:20Conclusion

Summary

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Tadução automática

An imaging-based method is described that can be used to identify S-phase and analyze cell cycle dynamics in the C. elegans hermaphrodite germline using the thymidine analog EdU. This method requires no transgenes and is compatible with immunofluorescent staining.

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Keywords: Cell Cycle AnalysisC. ElegansGermlineEdUThymidine AnalogImmunofluorescent StainingGerm Line Stem CellCell Cycle DynamicsAgingNutritionAltered GenotypesIdiodisprepationHematoxylin StainingSterile TechniqueM9 BufferE. ColiEdU SupplementationCentrifugationNematode Growth MediumPBSDissectionFixation

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