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寄生性锥虫锥虫锥虫的 f1-atpase 的分离

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Isolation of F₁-ATPase from the Parasitic Protist Trypanosoma brucei

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寄生性锥虫锥虫锥虫的 f1-atpase 的分离

DOI:

10.3791/58334-v

•

08:44 min

•

January 22, 2019

•

Ondřej Gahura, Alena Zíková²

¹Biology Centre,Czech Academy of Science, Institute of Parasitology, ²Faculty of Science,University of South Bohemia

Capítulos

00:04Título
00:57Preparation of Sub-Mitochondrial Particles
02:43Chloroform Release of F₁-ATPase from the Membrane
04:08Anion-Exchange Chromatography
05:34Size-Exclusion Chromatography
06:49Results: Two-Step Purification Results in Effective Isolation of F₁-ATPase
08:03Conclusion

Summary

Tadução automática

该方案描述了从锥虫培养阶段的 f1-atpase 的纯化.该程序产生了高度纯、均匀和活性的复合物, 适用于结构和酶学研究。

Tags

Keywords: F1-ATPase Trypanosoma Brucei Mitoplasts Protein Purification Mitochondrial Membrane Sonication Ultracentrifugation Chloroform Extraction Dounce Homogenizer

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