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Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
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JoVE Journal Biochimica
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

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08:44 min

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January 22, 2019

DOI:

10.3791/58334-v

DOI:
10.3791/58334-v

•

08:44 min
January 22, 2019

•

1 Views
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1Biology Centre,Czech Academy of Science, Institute of Parasitology, 2Faculty of Science,University of South Bohemia

Summary

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This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.

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Cite this Article

Gahura, O., Zíková, A. Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei. J. Vis. Exp. (143), e58334, doi:10.3791/58334 (2019).

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