Journal
/
Bioquímica
/
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

DOI:
10.3791/58334-v

•

08:44 min

•

January 22, 2019

•

,
1Biology Centre,Czech Academy of Science, Institute of Parasitology, 2Faculty of Science,University of South Bohemia

Capítulos

  • 00:04Título
  • 00:57Preparation of Sub-Mitochondrial Particles
  • 02:43Chloroform Release of F1-ATPase from the Membrane
  • 04:08Anion-Exchange Chromatography
  • 05:34Size-Exclusion Chromatography
  • 06:49Results: Two-Step Purification Results in Effective Isolation of F1-ATPase
  • 08:03Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.

Tags

Keywords: F1-ATPaseTrypanosoma BruceiMitoplastsProtein PurificationMitochondrial MembraneSonicationUltracentrifugationChloroform ExtractionDounce Homogenizer

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/58334?article_type=v

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
check_url/es/58334?article_type=v

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
check_url/es/58334?article_type=v

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
check_url/es/58334?article_type=v

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
check_url/es/58334?article_type=v

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
check_url/es/58334?article_type=v

Rapid Isolation of the Mitoribosome from HEK Cells
check_url/es/58334?article_type=v

Isolation of Tissue Extracellular Vesicles from the Liver
check_url/es/58334?article_type=v

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
check_url/es/58334?article_type=v

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
check_url/es/58334?article_type=v

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

Read Article