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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

11,836 Views

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12:06 min

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January 11, 2019

DOI:

10.3791/58297-v

DOI:
10.3791/58297-v

•

12:06 min
January 11, 2019

•

5 Views
, , , ,
1Center for Cancer Research,Massachusetts General Hospital, 2Department of Molecular Genetics,Weizmann Institute of Science

Tags

Human Primordial Germ Cell-like CellsPrimed-pluripotencyInduced Pluripotent Stem CellsGermline ToxicologyGenomic ImpairmentExtracellular MatrixSingle-cell SuspensionNaive Pluripotency4i MediumReproducibilityCell DensityCell Culture Model

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Mitsunaga, S., Shioda, K., Isselbacher, K. J., Hanna, J. H., Shioda, T. Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells. J. Vis. Exp. (143), e58297, doi:10.3791/58297 (2019).

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