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Effiziente und skalierbare gerichtete Differenzierung von klinisch kompatibel Hornhaut limbischen epithelialen Stammzellen aus menschlichen pluripotenten Stammzellen
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
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Effiziente und skalierbare gerichtete Differenzierung von klinisch kompatibel Hornhaut limbischen epithelialen Stammzellen aus menschlichen pluripotenten Stammzellen

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10:07 min

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October 24, 2018

DOI:

10.3791/58279-v

DOI:
10.3791/58279-v

•

10:07 min
October 24, 2018

•

3 Views
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1BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences,University of Tampere

Tags

Corneal Limbal Epithelial Stem CellsHuman Pluripotent Stem CellsFeeder-freeEmbryoid Body FormationBasal Induction MediumSB-505124Human Basic Fibroblast Growth FactorLimbal Stem Cell DeficiencyCorneal Cell Replacement Therapy

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Cite this Article

Hongisto, H., Vattulainen, M., Ilmarinen, T., Mikhailova, A., Skottman, H. Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (140), e58279, doi:10.3791/58279 (2018).

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