Journal
/
Imunologia e Infecção
/
Generering af rekombinant aviær Herpesvirus vektorer med CRISPR/Cas9 gen redigering
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Generering af rekombinant aviær Herpesvirus vektorer med CRISPR/Cas9 gen redigering

12,364 Views

•

12:21 min

•

January 07, 2019

DOI:

10.3791/58193-v

DOI:
10.3791/58193-v

•

12:21 min
January 07, 2019

•

4 Views
, , , , , , , ,
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Tags

CRISPR/Cas9Recombinant Avian HerpesvirusViral AntigensHVT GenomeRecombinant VaccinesGFP Expression CassetteCre-LoxP SystemCell SortingChick Embryo FibroblastsTransfectionHVT Virus StockPlaque Forming UnitsTrypsin-EDTACell SuspensionCell DensityCell SorterGFP-expressing Cells

Read Article

Cite this Article

Tang, N., Zhang, Y., Pedrera, M., Chang, P., Baigent, S., Moffat, K., Shen, Z., Nair, V., Yao, Y. Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing. J. Vis. Exp. (143), e58193, doi:10.3791/58193 (2019).

Download .ris file

Copy

Share Video

.

Copy