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Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

12,137 Views

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06:15 min

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August 18, 2018

DOI:

10.3791/58149-v

DOI:
10.3791/58149-v

•

06:15 min
August 18, 2018

•

2 Views
, , ,
1Department of Microbiology and Immunology and the Center for Human Immunology,University of Western Ontario, 2Institute of Infection and Global Health,University of Liverpool, 3Department of Respiratory Research,Aintree University Hospital NHS Foundation Trust

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EfferocytosisSingle-cell Fluorescence MicroscopyApoptotic Cell UptakeMacrophagesJurkat CellsNHS-BiotinCell-tracking DyeFITC-conjugated StreptavidinQuantificationMicroscope Optimization

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Taruc, K., Yin, C., Wootton, D. G., Heit, B. Quantification of Efferocytosis by Single-cell Fluorescence Microscopy. J. Vis. Exp. (138), e58149, doi:10.3791/58149 (2018).

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