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Forstærkning af nær fuld længde HIV-1 provirus for næste Generation Sequencing
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Genética
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JoVE Journal Genética
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Forstærkning af nær fuld længde HIV-1 provirus for næste Generation Sequencing

11,436 Views

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10:18 min

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October 16, 2018

DOI:

10.3791/58016-v

DOI:
10.3791/58016-v

•

10:18 min
October 16, 2018

•

4 Views
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1Centre for Virus Research, The Westmead Institute for Medical Research,University of Sydney

Tags

HIV-1 ProvirusesNext-generation SequencingSingle-cellAmplificationPCRGenetic CompositionReplication CompetentCell TypesLysis BufferSerial DilutionPositive ControlThermal Cycler

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Hiener, B., Eden, J., Horsburgh, B. A., Palmer, S. Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing. J. Vis. Exp. (140), e58016, doi:10.3791/58016 (2018).

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