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Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

DOI:
10.3791/57938-v

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08:33 min

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July 24, 2018

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, ,
1Department of Environmental Science, Center for Reservoir and Aquatic Systems Research,Baylor University, 2Institute of Biomedical Studies,Baylor University

Capítulos

  • 00:04Título
  • 01:46Exposing Fish to Chemical Treatment
  • 02:53Calibration of Video-tracking Parameters
  • 05:56Observation of Larval Fish Locomotor and Photomotor Behavior
  • 07:00Results: Caffeine Significantly Alters Zebrafish and Fathead Minnow Behavior
  • 07:43Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a protocol to examine larval zebrafish and fathead minnow locomotor activities and photomotor responses (PMR) using an automated tracking software. When incorporated in common toxicology bioassays, analyses of these behaviors provide a diagnostic tool to examine chemical bioactivity. This protocol is described using caffeine, a model neurostimulant.

Tags

Keywords: Fish ModelsBehavioral ResponseLarval FishCaffeineNeuro-stimulantEnvironmental Biomedical SciencesLocomotor ActivityPhotomotor ResponseZebrafishFathead MinnowExposure ChambersVideo Tracking

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