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実験で多年生の草の根、根、土壌微生物の分離と解析
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
DOI:

10:31 min

July 24, 2018

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Capítulos

  • 00:04Título
  • 00:36Plant Excavation, Soil Removal, and Bulk Soil Collection
  • 01:46Root and Rhizosphere Collection
  • 02:44Root Surface Sterilization
  • 03:54Processing Rhizosphere Samples
  • 05:02DNA Extraction and Soil Analysis Processing
  • 06:33DNA Extraction in 96-well Format
  • 07:45Rhizosphere Sample Loading
  • 08:47Results: Visualization and Statistical Methods Highlight Differences in Microbial Community Composition and Diversity Between Sample Types and Grass Species
  • 10:03Conclusion

Summary

Tadução automática

フィールドから植物の根の発掘だけでなく、土壌、根圏、endosphere に試料の加工は、DNA の抽出とデータ分析の方法を含め、詳細に説明します。本稿は、土壌、endosphere、および根圏内細菌叢解析の研究にこれらの技術を使用する他の研究室を有効にするのに設計されています。

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