00:36Plant Excavation, Soil Removal, and Bulk Soil Collection
01:46Root and Rhizosphere Collection
02:44Root Surface Sterilization
03:54Processing Rhizosphere Samples
05:02DNA Extraction and Soil Analysis Processing
06:33DNA Extraction in 96-well Format
07:45Rhizosphere Sample Loading
08:47Results: Visualization and Statistical Methods Highlight Differences in Microbial Community Composition and Diversity Between Sample Types and Grass Species
Excavation of plant roots from the field as well as processing of samples into endosphere, rhizosphere, and soil are described in detail, including DNA extraction and data analysis methods. This paper is designed to enable other laboratories to use these techniques for the study of soil, endosphere, and rhizosphere microbiomes.