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Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
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Umwelt
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JoVE Journal Umwelt
Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

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10:31 min

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July 24, 2018

DOI:

10.3791/57932-v

DOI:
10.3791/57932-v

•

10:31 min
July 24, 2018

•

166 Views
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1Department of Agronomy and Horticulture and Center for Plant Science Innovation,University of Nebraska-Lincoln, 2USDA-ARS, Wheat, Sorghum and Forage Research Unit,University of Nebraska-Lincoln East Campus

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Excavation of plant roots from the field as well as processing of samples into endosphere, rhizosphere, and soil are described in detail, including DNA extraction and data analysis methods. This paper is designed to enable other laboratories to use these techniques for the study of soil, endosphere, and rhizosphere microbiomes.

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McPherson, M. R., Wang, P., Marsh, E. L., Mitchell, R. B., Schachtman, D. P. Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments. J. Vis. Exp. (137), e57932, doi:10.3791/57932 (2018).

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