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代替の Pre-mrna スプライシング RNA In Situハイブリダイゼーション法によるマウス脳のセクションでの定量的解析

JoVE Journal
Genética

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JoVE Journal Genética

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

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代替の Pre-mrna スプライシング RNA In Situハイブリダイゼーション法によるマウス脳のセクションでの定量的解析

8,698 Views

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11:22 min

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August 26, 2018

DOI:

10.3791/57889-v

DOI:

10.3791/57889-v

•

11:22 min

August 26, 2018

•

6 Views

Xuan Guo^1,2, Yiqing Zhao^1,3, Hieu Nguyen¹, Tonghua Liu², Zhenghe Wang^1,3, Hua Lou^1,3,4

¹Department of Genetics and Genome Sciences,Case Western Reserve University, ²Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, ³Case Comprehensive Cancer Center,Case Western Reserve University, ⁴Center for RNA Science and Therapeutics,Case Western Reserve University

Summary

その場で交配 (っぽい) プロトコルマウス脳セクションの代替の Pre-mrna スプライシングパターンを検出する短いアンチセンスオリゴヌクレオチドを使用して記述されています。

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