Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
8,701 Views
•
11:22 min
•
August 26, 2018
DOI:
1Department of Genetics and Genome Sciences,Case Western Reserve University, 2Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, 3Case Comprehensive Cancer Center,Case Western Reserve University, 4Center for RNA Science and Therapeutics,Case Western Reserve University
Summary
Automatically generated
An in situ hybridization (ISH) protocol that uses short antisense oligonucleotides to detect alternative pre-mRNA splicing patterns in mouse brain sections is described.
Read Article
Cite this Article
Guo, X., Zhao, Y., Nguyen, H., Liu, T., Wang, Z., Lou, H. Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay. J. Vis. Exp. (138), e57889, doi:10.3791/57889 (2018).
Copy