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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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August 26, 2018
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1Department of Genetics and Genome Sciences,Case Western Reserve University, 2Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, 3Case Comprehensive Cancer Center,Case Western Reserve University, 4Center for RNA Science and Therapeutics,Case Western Reserve University
Summary
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An in situ hybridization (ISH) protocol that uses short antisense oligonucleotides to detect alternative pre-mRNA splicing patterns in mouse brain sections is described.
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Guo, X., Zhao, Y., Nguyen, H., Liu, T., Wang, Z., Lou, H. Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay. J. Vis. Exp. (138), e57889, doi:10.3791/57889 (2018).
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