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一种基于管尖的液滴微流化平台种子细胞的方法
JoVE Journal
Engenharia
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JoVE Journal Engenharia
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
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一种基于管尖的液滴微流化平台种子细胞的方法

DOI:
10.3791/57848-v

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06:50 min

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February 11, 2019

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, , , ,
1Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering,Eindhoven University of Technology, 2Institute for Immunity, Transplantation, and Infection, Beckman Center,Stanford University

Capítulos

  • 00:04Título
  • 00:42Lossless Cell Encapsulation
  • 03:04Emulsion Breaking and Cell Retrieval for Flow Cytometric Analysis
  • 04:30Results: Representative Cell Encapsulation in Droplets
  • 05:57Conclusion

Summary

Tadução automática

Tadução automática

本文提出了一种利用管道尖头对液滴微流体装置播种稀缺细胞群体的方案, 以提供更高的液滴细胞封装效率。

Tags

Pipette-tipCell SeedingDroplet MicrofluidicsSystems ImmunologyCellular HeterogeneityCommunicationRare CellsEncapsulationAqueous Droplet GenerationSurfactantFluorinated OilMineral OilPDMSSyringe PumpSample SolutionOil PhaseRPMI Medium

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