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A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
JoVE 杂志
工程学
This content is Free Access.
JoVE 杂志 工程学
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

DOI:
10.3791/57848-v

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06:50 min

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February 11, 2019

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, , , ,
1Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering,Eindhoven University of Technology, 2Institute for Immunity, Transplantation, and Infection, Beckman Center,Stanford University

Chapters

  • 00:04Title
  • 00:42Lossless Cell Encapsulation
  • 03:04Emulsion Breaking and Cell Retrieval for Flow Cytometric Analysis
  • 04:30Results: Representative Cell Encapsulation in Droplets
  • 05:57Conclusion

Summary

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This article presents a protocol for seeding scarce population of cells using pipette-tips to droplet microfluidic devices in order to provide higher encapsulation efficiency of cells in droplets.

Tags

Pipette-tipCell SeedingDroplet MicrofluidicsSystems ImmunologyCellular HeterogeneityCommunicationRare CellsEncapsulationAqueous Droplet GenerationSurfactantFluorinated OilMineral OilPDMSSyringe PumpSample SolutionOil PhaseRPMI Medium

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