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A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
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Engenharia
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JoVE Journal Engenharia
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

10,752 Views

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06:50 min

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February 11, 2019

DOI:

10.3791/57848-v

DOI:
10.3791/57848-v

•

06:50 min
February 11, 2019

•

7 Views
, , , ,
1Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering,Eindhoven University of Technology, 2Institute for Immunity, Transplantation, and Infection, Beckman Center,Stanford University

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This article presents a protocol for seeding scarce population of cells using pipette-tips to droplet microfluidic devices in order to provide higher encapsulation efficiency of cells in droplets.

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Pipette-tipCell SeedingDroplet MicrofluidicsSystems ImmunologyCellular HeterogeneityCommunicationRare CellsEncapsulationAqueous Droplet GenerationSurfactantFluorinated OilMineral OilPDMSSyringe PumpSample SolutionOil PhaseRPMI Medium

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Cite this Article

Sinha, N., Subedi, N., Wimmers, F., Soennichsen, M., Tel, J. A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms. J. Vis. Exp. (144), e57848, doi:10.3791/57848 (2019).

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