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大鼠外周全血细胞免疫表型和因子生成的单细胞分析
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
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大鼠外周全血细胞免疫表型和因子生成的单细胞分析

9,230 Views

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12:36 min

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June 26, 2018

DOI:

10.3791/57780-v

DOI:
10.3791/57780-v

•

12:36 min
June 26, 2018

•

2 Views
, , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2OMNI Biomarkers, Development Sciences,Genentech, 3Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Summary

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在这里, 我们描述了一个单细胞蛋白质组学方法, 以评估免疫表型和功能 (细胞因子诱导) 改变周围全血标本, 分析通过大规模细胞术。

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Single-cell AnalysisImmunophenotypeCytokine ProductionPeripheral Whole BloodMass CytometryAutoimmunityCell FrequencyCytokine ProductionSystems ImmunologyAutoimmune DiseaseSLEWhole Blood ProcessingRuxolitinibLyse/fix BufferCell RecoveryAntibody Staining

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Cite this Article

Baxter, R. M., Kong, D. S., Garcia-Perez, J. E., O'Gorman, W. E., Hsieh, E. W. Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry. J. Vis. Exp. (136), e57780, doi:10.3791/57780 (2018).

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