Rivista
/
Immunologia e infezioni
/
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

9,261 Views

•

12:36 min

•

June 26, 2018

DOI:

10.3791/57780-v

DOI:
10.3791/57780-v

•

12:36 min
June 26, 2018

•

2 Views
, , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2OMNI Biomarkers, Development Sciences,Genentech, 3Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Summary

Automatically generated

Here we describe a single-cell proteomic approach to evaluate immune phenotypic and functional (intracellular cytokine induction) alterations in peripheral whole blood samples, analyzed via mass cytometry.

Explore More Videos

Single-cell AnalysisImmunophenotypeCytokine ProductionPeripheral Whole BloodMass CytometryAutoimmunityCell FrequencyCytokine ProductionSystems ImmunologyAutoimmune DiseaseSLEWhole Blood ProcessingRuxolitinibLyse/fix BufferCell RecoveryAntibody Staining

Read Article

Cite this Article

Baxter, R. M., Kong, D. S., Garcia-Perez, J. E., O'Gorman, W. E., Hsieh, E. W. Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry. J. Vis. Exp. (136), e57780, doi:10.3791/57780 (2018).

Download .ris file

Copy

Share Video

.

Copy