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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
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JoVE 杂志 免疫与感染
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

DOI:
10.3791/57780-v

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12:36 min

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June 26, 2018

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, , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2OMNI Biomarkers, Development Sciences,Genentech, 3Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Chapters

  • 00:04Title
  • 01:10Stimulation and Processing of Peripheral Whole Blood
  • 05:27Barcode of Lysed/Fixed Blood Cells
  • 09:44Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
  • 11:50Conclusion

Summary

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Here we describe a single-cell proteomic approach to evaluate immune phenotypic and functional (intracellular cytokine induction) alterations in peripheral whole blood samples, analyzed via mass cytometry.

Tags

Keywords: Single-cell AnalysisImmunophenotypeCytokine ProductionPeripheral Whole BloodMass CytometryAutoimmunityCell FrequencyCytokine ProductionSystems ImmunologyAutoimmune DiseaseSLEWhole Blood ProcessingRuxolitinibLyse/fix BufferCell RecoveryAntibody Staining

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