Journal
/
Neurociência
/
激光捕获显微切割分离新鲜冷冻人体肠神经节的优化
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
Please note that all translations are automatically generated. Click here for the English version.

激光捕获显微切割分离新鲜冷冻人体肠神经节的优化

8,490 Views

•

08:28 min

•

June 14, 2018

DOI:

10.3791/57762-v

DOI:
10.3791/57762-v

•

08:28 min
June 14, 2018

•

5 Views
, ,
1Vanderbilt University Medical Center

Summary

Automatically generated

该协议的目的是获得高完整性的 RNA 样本从无固定的, 新鲜切除的人肠道组织, 利用激光捕获显微切割 (LCM) 分离。该协议包括制备人体肠道组织、cryosectioning、乙醇染色和脱水、LCM 和 RNA 提取的闪光冷冻样品。

Explore More Videos

Laser-capture MicrodissectionEnteric GangliaRNA ExtractionMyenteric PlexusTaeniae ColiDry Ice2-methylbutaneCryostatTFM

Read Article

Cite this Article

May-Zhang, A. A., Deal, K. K., Southard-Smith, E. M. Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue. J. Vis. Exp. (136), e57762, doi:10.3791/57762 (2018).

Download .ris file

Copy

Share Video

.

Copy