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JoVE Journal Neuroscience
Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
 

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

Article DOI: 10.3791/57762-v • 08:28 min • June 14th, 2018
June 14th, 2018 •
1, 1, 1
1Vanderbilt University Medical Center

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The goal of this protocol is to obtain high-integrity RNA samples from enteric ganglia isolated from unfixed, freshly-resected human intestinal tissue using laser capture microdissection (LCM). This protocol involves preparing flash-frozen samples of human intestinal tissue, cryosectioning, ethanolic staining and dehydration, LCM, and RNA extraction.

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Laser-Capture Microdissection Enteric Ganglia Fresh-frozen Human Tissue RNA Isolation Adipose Tissue Connective Tissue Serosa Myenteric Plexus Taeniae Coli Mesenteric Attachment Intestinal Tissue Mucosa Side Down Cutting Board Tissue Storage Solution Ice Crystals Disposable Plastic Mold Curved Forceps
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