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Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
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神经科学
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JoVE 杂志 神经科学
Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

DOI:
10.3791/57762-v

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08:28 min

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June 14, 2018

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, ,
1Vanderbilt University Medical Center

Chapters

  • 00:04Title
  • 00:29Preparation of Flash-Frozen Human Intestinal Tissue
  • 02:58Cryosectioning and Staining
  • 05:01Laser Capture Microdissection (LCM)
  • 07:05Results: Sequencing Data
  • 07:53Conclusion

Summary

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The goal of this protocol is to obtain high-integrity RNA samples from enteric ganglia isolated from unfixed, freshly-resected human intestinal tissue using laser capture microdissection (LCM). This protocol involves preparing flash-frozen samples of human intestinal tissue, cryosectioning, ethanolic staining and dehydration, LCM, and RNA extraction.

Tags

Laser-capture MicrodissectionEnteric GangliaRNA ExtractionMyenteric PlexusTaeniae ColiDry Ice2-methylbutaneCryostatTFM

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