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A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
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A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
Capítulos
- 00:04Título
- 00:54Extraction and Processing of Brain Tissue from Sprague-Dawley Rats
- 02:59Centrifugation
- 04:27Results: Analysis of Freshly Isolated Rat Brain Microvessel Samples
- 06:07Conclusion
Here, a method for isolation of rat brain microvessels and for the preparation of membrane samples is described. This protocol has the clear advantage of producing enriched microvessel samples with acceptable protein yield from individual animals. Samples can then be used for robust protein analyses at the brain microvascular endothelium.
