ジャーナル
/
神経科学
/
A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

DOI:
10.3791/57698-v

•

07:13 min

•

May 07, 2018

•

, , ,
1Department of Pharmacology, College of Medicine,University of Arizona, Tucson

章

  • 00:04標題
  • 00:54Extraction and Processing of Brain Tissue from Sprague-Dawley Rats
  • 02:59Centrifugation
  • 04:27Results: Analysis of Freshly Isolated Rat Brain Microvessel Samples
  • 06:07Conclusion

概要

自動翻訳

自動翻訳

Here, a method for isolation of rat brain microvessels and for the preparation of membrane samples is described. This protocol has the clear advantage of producing enriched microvessel samples with acceptable protein yield from individual animals. Samples can then be used for robust protein analyses at the brain microvascular endothelium.

タグ

Brain MicrovesselsBlood-brain BarrierRat BrainCerebral CortexProtease InhibitorBiochemical StudiesMembrane Isolation

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

The Vermicelli and Capellini Handling Tests: Simple quantitative measures of dexterous forepaw function in rats and mice

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

A Visual Description of the Dissection of the Cerebral Surface Vasculature and Associated Meninges and the Choroid Plexus from Rat Brain

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices

Read Article