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注射脂多糖对小鼠肠道屏障破坏后微生物源产物的模拟进入
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
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注射脂多糖对小鼠肠道屏障破坏后微生物源产物的模拟进入

DOI:
10.3791/57610-v

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08:24 min

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May 02, 2018

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, , , ,
1Department of Biomedicine,University of Basel, 2Division of Gastroenterology and Hepatology,University Hospital of Basel

Capítulos

  • 00:05Título
  • 00:57Intraperitoneal Injection of LPS to Mice
  • 01:53Tissue Sampling for Ribonucleic Acid (RNA) Extraction and Homogenization
  • 03:22RNA Extraction from Tissue
  • 05:47Digestion of Remaining DNA
  • 06:51Results: LPS Injection Induces the Expression of Pro-inflammatory Cytokines in C57Bl/6 Mice
  • 07:43Conclusion

Summary

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Tadução automática

本文提出了一种模拟肠道屏障破坏后细菌衍生化合物入口的协议。将低致死剂量的脂多糖系统地注入小鼠体内, 对24小时注射后进行监测。在脾、肝、结肠几个时间点上测定了促炎性细胞因子的表达。

Tags

LipopolysaccharideIntestinal Barrier BreachMicrobial-derived ProductsImmune ResponseInflammatory Bowel DiseasePeritoneal Cavity InjectionEndotoxemiaRNA IsolationOrgan Homogenization

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