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Immunology and Infection

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JoVE Journal Immunology and Infection
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
 

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Article DOI: 10.3791/57610-v • 08:24 min • May 2nd, 2018
May 2nd, 2018 •
1, 1, 1, 1, 1,2
1Department of Biomedicine, University of Basel, 2Division of Gastroenterology and Hepatology, University Hospital of Basel

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Summary
Automatic Translation

Here a protocol to mimic the entrance of bacterial-derived compounds after intestinal barrier breach is presented. A low sublethal dose of lipopolysaccharide was injected systemically into mice, which were monitored for 24 h post-injection. The expression of pro-inflammatory cytokines was determined at several time points in spleen, liver, and colon.

Tags

Lipopolysaccharide Injections Mice Microbial-derived Products Intestinal Barrier Breach Immune Responses Inflammatory Bowel Disease Intestinal Epithelial Permeability Defined Pump Laboratory Setting Procedure Demonstration Rachel Mak'Anyengo Berna Kaya Mouse Handling Injection Technique Peritoneal Cavity Endotoxemia Monitoring
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