Journal
/
Biologia do Desenvolvimento
/
培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
Please note that all translations are automatically generated. Click here for the English version.

培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

10,407 Views

•

08:15 min

•

May 02, 2018

DOI:

10.3791/57593-v

DOI:
10.3791/57593-v

•

08:15 min
May 02, 2018

•

9 Views
, ,
1Department of Biomedical Sciences,Cornell University, 2Department of Molecular Biology and Genetics,Cornell University

Summary

Automatically generated

在这里, 我们提出一项协议, 以量化卵泡在培养卵巢小鼠没有连续切片。用全器官免疫荧光和组织清除, 用光学切片代替物理切片。这种样品制备和可视化方法保持了器官的完整性, 促进了特定细胞的自动定量。

Explore More Videos

Whole Mount ImmunofluorescenceFollicle QuantificationCultured Mouse OvariesReproductive BiologyThree-dimensional ArchitectureDissectionIncubatorOvary Culture MediaImagingAutomated Analysis

Read Article

Cite this Article

Rinaldi, V. D., Bloom, J. C., Schimenti, J. C. Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries. J. Vis. Exp. (135), e57593, doi:10.3791/57593 (2018).

Download .ris file

Copy

Share Video

.

Copy