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Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
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Neurociência
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JoVE Journal Neurociência
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

9,750 Views

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09:50 min

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April 20, 2018

DOI:

10.3791/57526-v

DOI:
10.3791/57526-v

•

09:50 min
April 20, 2018

•

4 Views
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1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience,Université de Montréal, 3Department of pathology and cellular biology,Université de Montréal, 4Department of Pediatrics,Université de Montréal

Summary

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Here, we provide a low-cost and reliable method to generate electroporated brain organotypic slice cultures from mouse embryos suitable for confocal microscopy and live-imaging techniques.

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Ex Utero ElectroporationOrganotypic Slice CulturesEmbryonic Mouse BrainsLive-imagingMigrating GABAergic InterneuronsDevelopmental NeuroscienceGenetic Neurodevelopmental DisordersAutismEpilepsyBiosafety CabinetDissectionCultureNeural Based MediumTransfer PipetteBlack Wax

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Eid, L., Lachance, M., Hickson, G., Rossignol, E. Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons. J. Vis. Exp. (134), e57526, doi:10.3791/57526 (2018).

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