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JoVE Journal Neuroscience
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
 

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Article DOI: 10.3791/57526-v • 09:50 min • April 20th, 2018
April 20th, 2018 •
1,2, 1, 1,3, 1,2,4
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience, Université de Montréal, 3Department of pathology and cellular biology, Université de Montréal, 4Department of Pediatrics, Université de Montréal

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Here, we provide a low-cost and reliable method to generate electroporated brain organotypic slice cultures from mouse embryos suitable for confocal microscopy and live-imaging techniques.

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Ex Utero Electroporation Organotypic Slice Cultures Embryonic Mouse Brains Live-imaging Migrating GABAergic Interneurons Developmental Neurosciences Loss Of Function Specific Genes Brain Development Low Cost Easy To Perform Genetic Neurodevelopmental Disorders Autism Epilepsy Pathogenecity Specific Mutations Biosafety Cabinet Dissection And Culture 70% Ethanol Sterilize Instruments UV Light Collect Embryos
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