ジャーナル
/
神経科学
/
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

9,750 Views

•

09:50 min

•

April 20, 2018

DOI:

10.3791/57526-v

DOI:
10.3791/57526-v

•

09:50 min
April 20, 2018

•

4 Views
, , ,
1Centre de recherche du CHU Sainte-Justine, 2神経科学科,Université de Montréal, 3Department of pathology and cellular biology,Université de Montréal, 4小児科,Université de Montréal

概要

Automatically generated

Here, we provide a low-cost and reliable method to generate electroporated brain organotypic slice cultures from mouse embryos suitable for confocal microscopy and live-imaging techniques.

Explore More Videos

Ex Utero ElectroporationOrganotypic Slice CulturesEmbryonic Mouse BrainsLive-imagingMigrating GABAergic InterneuronsDevelopmental NeuroscienceGenetic Neurodevelopmental DisordersAutismEpilepsyBiosafety CabinetDissectionCultureNeural Based MediumTransfer PipetteBlack Wax

Read Article

Cite this Article

Eid, L., Lachance, M., Hickson, G., Rossignol, E. Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons. J. Vis. Exp. (134), e57526, doi:10.3791/57526 (2018).

Download .ris file

Copy

Share Video

.

Copy