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السريع وخط أنابيب سهلة "توليد طفرات نقطة الجينوم" في C. ايليجانس باستخدام كريسبر/Cas9 "ريبونوكليوبروتينس"
Capítulos
- 00:04Título
- 00:42sgRNA Target Selection and Homology-directed Repair Template Design
- 02:21Prepare Injection Mix and Injection Protocol
- 03:18Screen P0 Plates and Single mCherry(+) F1s
- 04:03Single Worm PCR and Genotyping
- 05:41Indentification and Sequence Verification of Edited Animals
- 06:34Results: Rapid CRISPR/Cas9 Generated Point Mutations in C. elegans sod-1
- 07:50Conclusion
