Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
7,387 Views
•
08:37 min
•
April 30, 2018
DOI:
Summary
Automatically generated
Here, we present a method to engineer the genome of C. elegans using CRISPR-Cas9 ribonucleoproteins and homology dependent repair templates.
Read Article
Cite this Article
Prior, H., MacConnachie, L., Martinez, J. L., Nicholl, G. C., Beg, A. A. A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins. J. Vis. Exp. (134), e57518, doi:10.3791/57518 (2018).
Copy