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Imunologia e Infecção
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Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

DOI:
10.3791/57514-v

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07:37 min

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May 22, 2018

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, , , , , ,
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology,Ministry of Agriculture, 2Tianjin Agricultural University

Capítulos

  • 00:04Título
  • 00:46Isolating SjEVs from Schistosoma japonicum Cultures
  • 03:00Characterization of EVs by Western Blotting and TEM
  • 04:01Labeling Schistosoma japonicum Extracellular Vesicles with PKH67 Followed by Cell Uptake
  • 06:15Results: Schistosoma japonicum Extracellular Vesicle Characterization
  • 07:17Conclusion

Summary

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Here, we present a protocol to describe extracellular vesicles (EVs) isolation in in vitro cultured medium from adult Schistosoma japonicum.

Tags

Keywords: Extracellular VesiclesSchistosoma JaponicumIn Vitro CultureIsolationPurificationCentrifugationDialysisPEG8000Western Blot

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