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Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
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Genética
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JoVE Journal Genética
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

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12:24 min

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May 05, 2018

DOI:

10.3791/57471-v

DOI:
10.3791/57471-v

•

12:24 min
May 05, 2018

•

3 Views
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1Department of Research,Hackensack University Medical Center, 2Department of Medical Sciences,Seton Hall University, 3Department of Epidemiology and Population Health,Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension,Hadassah – Hebrew University Medical Center

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Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.

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Loudig, O., Liu, C., Rohan, T., Ben-Dov, I. Z. Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens. J. Vis. Exp. (135), e57471, doi:10.3791/57471 (2018).

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