Rivista
/
Genetica
/
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

9,157 Views

•

12:24 min

•

May 05, 2018

DOI:

10.3791/57471-v

DOI:
10.3791/57471-v

•

12:24 min
May 05, 2018

•

3 Views
, , ,
1Department of Research,Hackensack University Medical Center, 2Department of Medical Sciences,Seton Hall University, 3Department of Epidemiology and Population Health,Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension,Hadassah – Hebrew University Medical Center

Summary

Automatically generated

Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.

Explore More Videos

MicroRNASequencingCDNA LibraryFormalin-fixed Paraffin-embeddedRNAProtocolLigationAdapterPrecipitationCentrifugation

Read Article

Cite this Article

Loudig, O., Liu, C., Rohan, T., Ben-Dov, I. Z. Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens. J. Vis. Exp. (135), e57471, doi:10.3791/57471 (2018).

Download .ris file

Copy

Share Video

.

Copy