Journal
/
Neurociência
/
创新 RNA原位检测技术在 D1-Alpha 鼠伏隔核中多巴胺受体的鉴定
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology
Please note that all translations are automatically generated. Click here for the English version.

创新 RNA原位检测技术在 D1-Alpha 鼠伏隔核中多巴胺受体的鉴定

DOI:
10.3791/57444-v

•

07:25 min

•

March 27, 2018

•

, , , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Capítulos

  • 00:04Título
  • 01:00Preparation of Fresh Frozen Brain Sections
  • 02:35RNA In Situ Hybridization Fluorescent Multiplex Assay
  • 05:12Results: Drd1α RNA Level in Nucleus Accumbens Region of F344/N Rats
  • 06:35Conclusion

Summary

Tadução automática

Tadução automática

在伏隔核中多巴胺 D1-alpha 受体的鉴定对于澄清中枢神经系统疾病中 D1 受体功能障碍至关重要。我们进行了一个新的 rna 原位杂交检测, 以可视化单个 RNA 分子在特定的大脑区域。

Tags

RNA In-situ HybridizationDopamine D1-alpha ReceptorNucleus AccumbensCentral Nervous System DiseaseDopaminergic DysfunctionTarget MRNABrain SectioningPre-treatmentMultiplex Assay

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

同时在啮齿动物脑功能磁共振成像和电

VisioTracker，创新的自动化到动眼神经分析方法

啮齿动物的中枢神经系统药物直接脑室交货

检测持久性病毒的DNA在神经组织​​中的荧光的基因组与成绩单原位杂交结合免疫组化染色

在啮齿动物中枢神经系统的体内光遗传学刺激

在脑切片检测轴突本地化mRNAs的高空间分辨率<em>原位</em>杂交

接口3D工程神经细胞微电极阵列：一种创新<em>体外</em>实验模型

体内基因转移到雪旺细胞在啮齿动物坐骨神经电穿孔

生活多巴胺能神经元可靠的鉴定中脑培养使用RNA测序和TH-启动子驱动的EGFP表达

通过RNA定位蝗虫天线中的气味受体基因<em>原位</em>杂交

Read Article