Identification of dopamine D1-alpha receptor in the nucleus accumbens is critical for clarifying D1 receptor dysfunction during a central nervous system disease. We performed a novel RNA in situ hybridization assay to visualize single RNA molecules in a specific brain area.
Li, H., Illenberger, J. M., McLaurin, K. A., Mactutus, C. F., Booze, R. M. Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology. J. Vis. Exp. (133), e57444, doi:10.3791/57444 (2018).