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Biologia do Desenvolvimento

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成人耳皮肤全安装共焦显微术: 一种研究神经血管分支形态发生和免疫细胞分布的模型系统

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

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成人耳皮肤全安装共焦显微术: 一种研究神经血管分支形态发生和免疫细胞分布的模型系统

DOI:

10.3791/57406-v

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08:42 min

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March 29, 2018

•

Tomoko Yamazaki², Wenling Li, Yoh-Suke Mukouyama

¹Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, ²Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Capítulos

00:04Título
01:03Adult Mouse Skin Collection
02:50Whole-mount Immunohistochemical Staining of Mouse Ear Skin
05:16Mounting Ear Skin on Slide
06:42Confocal Microscopy
07:13Results: Neuro-vascular Branching Morphogenesis and Immune Cell Distribution in Adult Ear Skin
07:56Conclusion

Summary

Tadução automática

在这里, 我们描述了一个高分辨率全安装成像方法在整个成年小鼠耳皮肤, 这使我们能够想象分支形态发生和模式的周围神经和血管, 以及免疫细胞分布。

Tags

Keywords: Whole-mount Confocal Microscopy Ear Skin Neuro-vascular Branching Morphogenesis Immune Cell Distribution Peripheral Nerves Blood Vessels Immunohistochemistry Skin Preparation 3D Visualization Normal And Pathological Conditions

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