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Biologia do Desenvolvimento
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Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

DOI:
10.3791/57406-v

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08:42 min

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March 29, 2018

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, ,
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Capítulos

  • 00:04Título
  • 01:03Adult Mouse Skin Collection
  • 02:50Whole-mount Immunohistochemical Staining of Mouse Ear Skin
  • 05:16Mounting Ear Skin on Slide
  • 06:42Confocal Microscopy
  • 07:13Results: Neuro-vascular Branching Morphogenesis and Immune Cell Distribution in Adult Ear Skin
  • 07:56Conclusion

Summary

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Here, we describe a high resolution whole-mount imaging method in the entire adult mouse ear skin, which enables us to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels, as well as immune cell distribution.

Tags

Keywords: Whole-mountConfocal MicroscopyEar SkinNeuro-vascular BranchingMorphogenesisImmune Cell DistributionPeripheral NervesBlood VesselsImmunohistochemistrySkin Preparation3D VisualizationNormal And Pathological Conditions

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