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Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
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Neurociência
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JoVE Journal Neurociência
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

DOI:
10.3791/57353-v

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06:49 min

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March 21, 2018

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, , , , , ,
1Department of Biology, MS-0314,University of Nevada, 2Integrated Neuroscience Graduate Program,University of Nevada

Capítulos

  • 00:04Título
  • 00:38Building Behavioral Arena and Preparing Optogenetic Stimulation Hardware
  • 02:49Preparing Drosophila Larvae Behavior Analyses
  • 04:36Behavior Assay
  • 05:42Results: Specific ORN Activation
  • 06:17Conclusion

Summary

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This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software.

Tags

Drosophila LarvaOptogenetic StimulationOlfactory NeuronsBehavior ArenaCCD CameraInfrared LEDLED PlatformRaspberry PiCsChrimsonORX-GAL4

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