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Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

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06:49 min

March 21, 2018

DOI:

06:49 min
March 21, 2018

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Summary

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This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software.

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