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新鲜人脑组织中毛细血管的分离
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Neurociência
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JoVE Journal Neurociência
Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
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新鲜人脑组织中毛细血管的分离

DOI:
10.3791/57346-v

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06:35 min

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September 12, 2018

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, , , , , , , ,
1Sanders-Brown Center on Aging, Department of Pharmacology and Nutritional Sciences,University of Kentucky, 2Department of Pharmaceutical Sciences, College of Pharmacy,University of Kentucky, 3Department of Neuroscience,University of Kentucky

Capítulos

  • 00:04Título
  • 00:34Brain Sample Preparation
  • 02:41Filtration and Capillary Collection
  • 05:14Results: Isolated Human Brain Capillary
  • 05:40Conclusion

Summary

Tadução automática

Tadução automática

从人脑组织中分离出的脑毛细血管可作为研究生理和病理生理学条件下屏障功能的临床前模型。在这里, 我们提出了一个优化的协议, 以隔离脑毛细血管与新鲜的人脑组织。

Tags

Cerebral CapillariesBlood-brain BarrierIsolationHomogenizationFicoll Density GradientFiltrationCentrifugation

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