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Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
Capítulos
- 00:04Título
- 00:43Synchronous Culture of Cyanobacteria and Monitoring by Fluorescence Microscopy
- 02:31Sample Freezing and Cryo-HVET
- 05:16Tomographic Reconstruction and Segmentation of the Feature of Interest
- 07:20Results: Observations of Compacted DNA in Cyanobacteria
- 08:36Conclusion
This protocol describes how to visualize the transient DNA compaction in cyanobacteria. Synchronous cultivation, monitoring by fluorescence microscopy, rapid freezing, and high voltage cryo-electron tomography are used. A protocol for these methodologies is presented, and future applications and developments are discussed.
