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高压低温电子层析成像对蓝藻 DNA 压实的可视化研究
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
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高压低温电子层析成像对蓝藻 DNA 压实的可视化研究

8,846 Views

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09:47 min

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July 17, 2018

DOI:

10.3791/57197-v

DOI:
10.3791/57197-v

•

09:47 min
July 17, 2018

•

2 Views
,
1National Institute for Physiological Sciences, 2Graduate School of Science and Engineering,Saitama University

Summary

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该协议描述了如何可视化蓝藻中的瞬态 DNA 压缩。采用同步培养、荧光显微镜、快速冷冻、高压低温电子层析成像等技术进行监测。提出了这些方法的一个协议, 并讨论了未来的应用和发展。

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Cite this Article

Murata, K., Kaneko, Y. Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography. J. Vis. Exp. (137), e57197, doi:10.3791/57197 (2018).

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