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Paraffine insluiten en dunne afdelen van microbiële kolonie Biofilms voor microscopische analyse
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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Paraffine insluiten en dunne afdelen van microbiële kolonie Biofilms voor microscopische analyse

13,761 Views

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08:52 min

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March 23, 2018

DOI:

10.3791/57196-v

DOI:
10.3791/57196-v

•

08:52 min
March 23, 2018

•

2 Views
, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Tags

Paraffin EmbeddingThin SectioningMicrobial Colony BiofilmsMicroscopic AnalysisGene ExpressionFluorescent ProteinBiofilm ArchitectureExopolymeric SubstancesAgar Tryptone SolutionP. AeruginosaColony BiofilmsEmbedding CassetteFixativePBSMicroscopy

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Cornell, W. C., Morgan, C. J., Koyama, L., Sakhtah, H., Mansfield, J. H., Dietrich, L. E. Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis. J. Vis. Exp. (133), e57196, doi:10.3791/57196 (2018).

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