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Paraffine insluiten en dunne afdelen van microbiële kolonie Biofilms voor microscopische analyse
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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Paraffine insluiten en dunne afdelen van microbiële kolonie Biofilms voor microscopische analyse

13,832 Views

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08:52 min

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March 23, 2018

DOI:

10.3791/57196-v

DOI:
10.3791/57196-v

•

08:52 min
March 23, 2018

•

7 Views
, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Summary

Automatically generated

We beschrijven fixatie, paraffine insluiten en dunne vectorafbeeldingsbestanden technieken voor microbiële kolonie biofilms. In bereid monsters, kunnen biofilm substructuur en verslaggever expressiepatronen worden gevisualiseerd door microscopie.

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Cite this Article

Cornell, W. C., Morgan, C. J., Koyama, L., Sakhtah, H., Mansfield, J. H., Dietrich, L. E. Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis. J. Vis. Exp. (133), e57196, doi:10.3791/57196 (2018).

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