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Methode voor het efficiënt uiteinde en zuivering van de Chemoreceptor-Ligand bindend domein

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

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Methode voor het efficiënt uiteinde en zuivering van de Chemoreceptor-Ligand bindend domein

17,354 Views

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14:25 min

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December 12, 2017

DOI:

10.3791/57092-v

DOI:

10.3791/57092-v

•

14:25 min

December 12, 2017

•

5 Views

Mayra A. Machuca², Anna Roujeinikova^2,3

¹Infection and Immunity Program,Monash Biomedicine Discovery Institute, ²Department of Microbiology,Monash University, ³Department of Biochemistry and Molecular Biology,Monash University

Tags

Chemoreceptor Ligand Binding Domain Refolding Purification Inclusion Bodies E. Coli Protein Expression IPTG Induction Cell Lysis Homogenization Centrifugation SDS-PAGE Buffer Exchange Solubilization Protein Purification

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